1-β-D-阿拉伯呋喃糖基胸腺嘧啶对爱泼斯坦-巴尔病毒合成的抑制作用。

Inhibitory effect of 1-beta-D-arabinofuranosylthymine on synthesis of Epstein-Barr virus.

作者信息

Yonemura K, Sairenji T, Hinuma Y

出版信息

Microbiol Immunol. 1981;25(6):557-63. doi: 10.1111/j.1348-0421.1981.tb00057.x.

DOI:10.1111/j.1348-0421.1981.tb00057.x

Abstract

The effect of 1-beta-D-arabinofuranosylthymine (ara-T) on cell growth and synthesis of Epstein-Barr virus (EBV) in human lymphoblastoid cell lines was determined. The growth of P3HR-1 cells was not inhibited by 1 microgram of the drug per ml; however, infectious virus production was strongly inhibited and was accompanied by decreased expression of early antigen (EA) and viral capsid antigen (VCA). The ability of 12-O-tetradecanoylphorbol-13-acetate or n-butyric acid to induce synthesis of VCA, but not EA, in P3HR-1 cells was inhibited by ara-T. Similarly, VCA synthesis but not EA synthesis was inhibited by ara-T in Jijoye cells superinfected with the P3HR-1 strain of EBV. The results suggest that ara-T has a specific inhibitory action against EBV replication.

摘要

测定了1-β-D-阿拉伯呋喃糖基胸腺嘧啶（ara-T）对人淋巴母细胞系中细胞生长及爱泼斯坦-巴尔病毒（EBV）合成的影响。每毫升含1微克该药物时，P3HR-1细胞的生长未受抑制；然而，传染性病毒的产生受到强烈抑制，并伴有早期抗原（EA）和病毒衣壳抗原（VCA）表达的降低。ara-T抑制了12-O-十四酰佛波醇-13-乙酸酯或正丁酸在P3HR-1细胞中诱导VCA合成的能力，但不影响EA的合成。同样，ara-T抑制了感染EBV P3HR-1株的Jijoye细胞中VCA的合成，但不影响EA的合成。结果表明ara-T对EBV复制具有特异性抑制作用。