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使用对氚敏感胶片的受体放射自显影术:计算机化密度测定的潜力。

Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry.

作者信息

Palacios J M, Niehoff D L, Kuhar M J

出版信息

Neurosci Lett. 1981 Sep 1;25(2):101-5. doi: 10.1016/0304-3940(81)90315-3.

DOI:10.1016/0304-3940(81)90315-3
PMID:6269032
Abstract

Benzodiazepine receptors mounted tissue sections of brain were localized by autoradiography using a tritium-sensitive sheet film (3H-Ultrofilm, LKB). The use of the film had many advantages compared to a previous technique utilizing emulsion-coated coverslips. A major advantage is the adaptability of the film to microdensitometric techniques for measurement of receptors. The densitometry can be carried out by computerized, color-coded image analysis.

摘要

利用氚敏感片膜(LKB公司的3H-Ultrofilm)通过放射自显影术对安装在脑组织结构切片上的苯二氮䓬受体进行定位。与先前使用涂有乳胶的盖玻片的技术相比,使用这种片膜有许多优点。一个主要优点是该片膜适用于微密度测定技术来测量受体。密度测定可通过计算机化的彩色编码图像分析来进行。

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