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覆盖鸡卵黄蛋白原基因的基因组克隆的分离与鉴定。

Isolation and characterization of genomic clones covering the chicken vitellogenin gene.

作者信息

Arnberg A C, Meijlink F C, Mulder J, van Bruggen E F, Gruber M, Geert A B

出版信息

Nucleic Acids Res. 1981 Jul 24;9(14):3271-86. doi: 10.1093/nar/9.14.3271.

DOI:10.1093/nar/9.14.3271
PMID:6269078
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327351/
Abstract

A series of overlapping recombinant clones, which cover the vitellogenin gene, has been isolated from a phage-lambda linked chicken gene library. The DNA of the overlapping clones spans 28 kb of contiguous DNA sequences in the chicken genome. Electron microscopic analysis of hybrids between vitellogenin mRNA and the genomic clones indicates that the chicken vitellogenin gene has a length of approximately 22 kb, about 3.8 times the size of the mRNA. The mRNA sequence is interrupted by at least 33 intervening sequences (introns). Comparison with the vitellogenin gene A2 from Xenopus laevis (Wahli et al., 1980, Cell 20: 107-117) indicates conservation of the number and length of the exons during evolution. Heteroduplex analysis reveals a short stretch of sequence homology between the genes from chicken and frog.

摘要

从一个与λ噬菌体相连的鸡基因文库中分离出了一系列覆盖卵黄生成素基因的重叠重组克隆。这些重叠克隆的DNA在鸡基因组中跨越了28 kb的连续DNA序列。对卵黄生成素mRNA与基因组克隆之间的杂交体进行电子显微镜分析表明，鸡卵黄生成素基因长度约为22 kb，约为mRNA大小的3.8倍。mRNA序列被至少33个间隔序列（内含子）中断。与非洲爪蟾的卵黄生成素基因A2（Wahli等人，1980年，《细胞》20: 107 - 117）进行比较表明，在进化过程中外显子的数量和长度具有保守性。异源双链分析揭示了鸡和蛙的基因之间有一小段序列同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/6be84c4ba957/nar00407-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/3867e1f395df/nar00407-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/4c4015ed415a/nar00407-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/914d5ac5432a/nar00407-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/6be84c4ba957/nar00407-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/3867e1f395df/nar00407-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/4c4015ed415a/nar00407-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/914d5ac5432a/nar00407-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/6be84c4ba957/nar00407-0070-a.jpg

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本文引用的文献

1
Comparative analysis of the structural organization of two closely related vitellogenin genes in X. laevis.非洲爪蟾中两个密切相关的卵黄蛋白原基因结构组织的比较分析。
Cell. 1980 May;20(1):107-17. doi: 10.1016/0092-8674(80)90239-1.
2
Identificiation, purification, and characterization of two distinct avian vitellogenins.两种不同禽类卵黄生成素的鉴定、纯化及特性分析
Biochemistry. 1980 Apr 15;19(8):1557-63. doi: 10.1021/bi00549a004.
3
Some yeast mitochondrial RNAs are circular.一些酵母线粒体RNA是环状的。
肝脏雌激素反应性卵黄蛋白原和载脂蛋白-VLDLII基因转录起始位点之前区域的序列同源性。
Nucleic Acids Res. 1984 Nov 26;12(22):8611-26. doi: 10.1093/nar/12.22.8611.
4
Evolution of vitellogenin genes: comparative analysis of the nucleotide sequences downstream of the transcription initiation site of four Xenopus laevis and one chicken gene.卵黄蛋白原基因的进化：对四种非洲爪蟾和一种鸡基因转录起始位点下游核苷酸序列的比较分析。
Nucleic Acids Res. 1984 Nov 26;12(22):8595-609. doi: 10.1093/nar/12.22.8595.
5
Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.非洲爪蟾的卵黄蛋白原B2基因：分离、体外转录及其与其他卵黄蛋白原基因的关系
Nucleic Acids Res. 1983 May 25;11(10):2979-97. doi: 10.1093/nar/11.10.2979.
6
Methylation of the chicken vitellogenin gene: influence of estradiol administration.鸡卵黄生成素基因的甲基化：雌二醇给药的影响
Nucleic Acids Res. 1983 Mar 11;11(5):1361-73. doi: 10.1093/nar/11.5.1361.
7
Comparative analysis of Xenopus tropicalis and Xenopus laevis vitellogenin gene sequences.热带爪蟾和非洲爪蟾卵黄蛋白原基因序列的比较分析。
Nucleic Acids Res. 1982 Mar 11;10(5):1515-33. doi: 10.1093/nar/10.5.1515.
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Sequence homologies within the 5' end region of the estrogen-controlled vitellogenin gene in Xenopus and chicken.非洲爪蟾和鸡中雌激素调控的卵黄蛋白原基因5'端区域内的序列同源性。
EMBO J. 1983;2(12):2271-9. doi: 10.1002/j.1460-2075.1983.tb01734.x.
9
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