覆盖鸡卵黄蛋白原基因的基因组克隆的分离与鉴定。

Isolation and characterization of genomic clones covering the chicken vitellogenin gene.

作者信息

Arnberg A C, Meijlink F C, Mulder J, van Bruggen E F, Gruber M, Geert A B

出版信息

Nucleic Acids Res. 1981 Jul 24;9(14):3271-86. doi: 10.1093/nar/9.14.3271.

DOI:10.1093/nar/9.14.3271

PMID:6269078

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327351/

Abstract

A series of overlapping recombinant clones, which cover the vitellogenin gene, has been isolated from a phage-lambda linked chicken gene library. The DNA of the overlapping clones spans 28 kb of contiguous DNA sequences in the chicken genome. Electron microscopic analysis of hybrids between vitellogenin mRNA and the genomic clones indicates that the chicken vitellogenin gene has a length of approximately 22 kb, about 3.8 times the size of the mRNA. The mRNA sequence is interrupted by at least 33 intervening sequences (introns). Comparison with the vitellogenin gene A2 from Xenopus laevis (Wahli et al., 1980, Cell 20: 107-117) indicates conservation of the number and length of the exons during evolution. Heteroduplex analysis reveals a short stretch of sequence homology between the genes from chicken and frog.

摘要

从一个与λ噬菌体相连的鸡基因文库中分离出了一系列覆盖卵黄生成素基因的重叠重组克隆。这些重叠克隆的DNA在鸡基因组中跨越了28 kb的连续DNA序列。对卵黄生成素mRNA与基因组克隆之间的杂交体进行电子显微镜分析表明，鸡卵黄生成素基因长度约为22 kb，约为mRNA大小的3.8倍。mRNA序列被至少33个间隔序列（内含子）中断。与非洲爪蟾的卵黄生成素基因A2（Wahli等人，1980年，《细胞》20: 107 - 117）进行比较表明，在进化过程中外显子的数量和长度具有保守性。异源双链分析揭示了鸡和蛙的基因之间有一小段序列同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f950/327351/3867e1f395df/nar00407-0063-a.jpg

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Cell. 1980 May;20(1):107-17. doi: 10.1016/0092-8674(80)90239-1.

Identificiation, purification, and characterization of two distinct avian vitellogenins.

Biochemistry. 1980 Apr 15;19(8):1557-63. doi: 10.1021/bi00549a004.

Some yeast mitochondrial RNAs are circular.

Cell. 1980 Feb;19(2):313-9. doi: 10.1016/0092-8674(80)90505-x.

Recombinant plasmids containing avian vitellogenin structural gene sequences derived from complementary DNA.

Biochim Biophys Acta. 1980;606(1):34-46. doi: 10.1016/0005-2787(80)90095-7.

Coordinate regulation of two estrogen-dependent genes in avian liver.

Proc Natl Acad Sci U S A. 1980 Aug;77(8):4474-8. doi: 10.1073/pnas.77.8.4474.

Characterization of cloned complementary DNA covering more than 6000 nucleotides (97%) of avian vitellogenin mRNA.

Eur J Biochem. 1980 Dec;112(3):443-50. doi: 10.1111/j.1432-1033.1980.tb06106.x.

Detection of specific sequences among DNA fragments separated by gel electrophoresis.

J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.

Kinetics of estrogen induction of Xenopus laevis vitellogenin messenger RNA as measured by hybridization to complementary DNA.

J Biol Chem. 1977 Dec 10;252(23):8428-34.

A physical map of the DNA regions flanking the rabbit beta-globin gene.

Cell. 1977 Oct;12(2):429-39. doi: 10.1016/0092-8674(77)90119-2.

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

J Mol Biol. 1977 Jun 15;113(1):237-51. doi: 10.1016/0022-2836(77)90052-3.

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覆盖鸡卵黄蛋白原基因的基因组克隆的分离与鉴定。

Isolation and characterization of genomic clones covering the chicken vitellogenin gene.

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覆盖鸡卵黄蛋白原基因的基因组克隆的分离与鉴定。

Isolation and characterization of genomic clones covering the chicken vitellogenin gene.

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出版信息

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