Betz H
Brain Res. 1981 Oct 26;223(1):190-4. doi: 10.1016/0006-8993(81)90822-2.
The activity of choline acetyltransferase (CAT) was investigated in primary cultures of retina from 8-day-old chick embryos. After the second day in culture, the total and specific activities of the enzyme decreased by more than 50%. Cultivation of the cells in 56 mM K+ or 59 microgram/ml of veratrine retarded the loss of CAT activity up to days 3-5 in vitro. If the addition of 56 mM K+ or 59 microgram/ml of veratrine was delayed until the third day of culture, these agents had no effect on the remaining CAT activity. These results suggest that the survival in culture of a part of the cholinergic retinal neurons requires membrane depolarization.
在8日龄鸡胚视网膜原代培养物中研究了胆碱乙酰转移酶（CAT）的活性。培养第二天后，该酶的总活性和比活性下降超过50%。在56 mM K+或59微克/毫升藜芦碱中培养细胞，可将CAT活性的丧失延缓至体外培养的第3 - 5天。如果将56 mM K+或59微克/毫升藜芦碱的添加推迟到培养的第三天，这些试剂对剩余的CAT活性没有影响。这些结果表明，一部分胆碱能视网膜神经元在培养中的存活需要膜去极化。
