Slaga T J, Bracken W M, Dresner S, Levin W, Yagi H, Jerina D M, Conney A H
Cancer Res. 1978 Mar;38(3):678-81.
The skin tumor-initiating activities of the 12 isomeric phenols of benzo(a)pyrene (BP) were determined in mice by use of a two-stage system of tumorigenesis. 11-Hydroxybenzo(a)pyrene was moderately active, whereas 2-hydroxybenzo(a)pyrene and BP were strong tumor initiators when applied topically to CD-1 mice and followed by twice-weekly applications of the promoter 12-O-tetradecanoylphorbol-13-acetate. 1-, 3-, 4-, 5-, 6-, 7-, 8-, 9-, 10-, and 12-hydroxybenzo(a)pyrene had less than 5% of the tumor-initiating activity of BP when the data were expressed as papillomas per mouse. After 30 weeks of promotion, the number of papillomas per mouse was 8.4, 8.5, and 2.8, respectively, for the animals treated with BP, 2-hydroxybenzo(a)pyrene, and 11-hydroxybenzo(a)pyrene. A 5-week latency period before the appearance of the first tumor was observed after the application of either 2-hydroxybenzo(a)pyrene or BP, whereas a slightly longer latency period of 7 weeks was observed following application of 11-hydroxybenzo(a)pyrene. The time required for 50% of the animals to develop tumors was 13 weeks for animals treated with BP and 15 weeks for animals treated with 2- or 11-hydroxybenzo(a)pyrene.
利用肿瘤发生的两阶段系统，在小鼠中测定了苯并(a)芘（BP）的12种异构体酚类的皮肤肿瘤起始活性。当局部应用于CD-1小鼠，随后每周两次应用启动子12-O-十四酰佛波醇-13-乙酸酯时，11-羟基苯并(a)芘具有中等活性，而2-羟基苯并(a)芘和BP是强肿瘤起始剂。当数据表示为每只小鼠的乳头状瘤时，1-、3-、4-、5-、6-、7-、8-、9-、10-和12-羟基苯并(a)芘的肿瘤起始活性低于BP的5%。在促进30周后，用BP、2-羟基苯并(a)芘和11-羟基苯并(a)芘处理的动物每只小鼠的乳头状瘤数量分别为8.4、8.5和2.8。在应用2-羟基苯并(a)芘或BP后，观察到在第一个肿瘤出现前有5周的潜伏期，而在应用11-羟基苯并(a)芘后观察到稍长的7周潜伏期。用BP处理的动物50%发生肿瘤所需的时间为13周，用2-或11-羟基苯并(a)芘处理的动物为15周。
