Harris S I, Balaban R S, Barrett L, Mandel L J
J Biol Chem. 1981 Oct 25;256(20):10319-28.
Cellular oxygen consumption was monitored during stimulation and inhibition of the Na+- and K+-dependent adenosine triphosphatase in a suspension of intact tubules isolated from the rabbit renal cortex. Respiratory rates were compared to the ADP-stimulated respiratory rate (state 3 rate) obtained in mitochondria released directly from the renal tubules by digitonin shock. At 37 degrees C, in the presence of NADH-linked substrates and fats, isolated renal cells respire at 50 to 60% of the state 3 rate. Inhibition of the (Na+,K+)-ATPase with the cardiac glycoside, ouabain, results in a decline in respiration to 25 to 30% of the state 3 rate. Stimulation of the (Na+,K+)-ATPase produced as a result of nystatin-mediated dissipation of plasma membrane Na+ and K+ gradients results in increased respiration with an oxygen consumption rate characteristic of optimal ATP synthesis (state 3). The relationship between metabolic substrate regimen, mitochondrial respiratory capacity, and cellular energy demand is examined in the context of these findings.
在对从兔肾皮质分离出的完整肾小管悬浮液中钠钾依赖型三磷酸腺苷酶进行刺激和抑制的过程中，监测细胞耗氧量。将呼吸速率与通过洋地黄皂苷休克直接从肾小管释放的线粒体中获得的二磷酸腺苷刺激呼吸速率（状态3速率）进行比较。在37摄氏度下，在存在与烟酰胺腺嘌呤二核苷酸（NADH）相关的底物和脂肪的情况下，分离出的肾细胞呼吸速率为状态3速率的50%至60%。用强心苷哇巴因抑制（钠，钾）-三磷酸腺苷酶会导致呼吸速率下降至状态3速率的25%至30%。由于制霉菌素介导的质膜钠钾梯度消散而产生的（钠，钾）-三磷酸腺苷酶刺激会导致呼吸增加，其耗氧率具有最佳三磷酸腺苷合成（状态3）的特征。在这些研究结果的背景下，研究了代谢底物方案、线粒体呼吸能力和细胞能量需求之间的关系。
