Tucker R F, Babul J, Stellwagen E
J Biol Chem. 1981 Nov 10;256(21):10993-8.
The chromatographic behavior of a heterogeneous protein mixture and of a series of homogeneous proteins on the immobilized dye tetraiodofluorescein has been observed and analyzed. Less than 6%, of the millimolar concentration of dye immobilized to a porous agarose matrix is accessible to protein. The affinity of a protein for immobilized dye is dramatically increased by insertion of apolar spacer atoms between the dye and the matrix. Dye columns constructed with a 9-atom spacer can be used to advantage for the retention and competitive elution of proteins not found previously amenable to dye chromatography.
已观察并分析了异质蛋白质混合物以及一系列均质蛋白质在固定化染料四碘荧光素上的色谱行为。固定在多孔琼脂糖基质上的染料，其毫摩尔浓度中只有不到6%可与蛋白质接触。通过在染料和基质之间插入非极性间隔原子，蛋白质对固定化染料的亲和力会显著增加。用9个原子的间隔物构建的染料柱可有效地用于保留和竞争性洗脱以前不适用于染料色谱法的蛋白质。
