Rajgopal S, Vijayalakshmi M A
J Chromatogr. 1984 Dec 19;315:175-84. doi: 10.1016/s0021-9673(01)90735-6.
Tetraiodofluorescein binds to luciferase in an apparently competitive manner, according to kinetic inactivation studies. Among the metal ions studied, Cu2+ and Ni2+ are the only two of the first row transition series that enhance enzyme inactivation by the dye. Mg2+, Co2+ and Mn2+ seem to have a protective effect whereas Zn2+ prevents enzyme inactivation and even enhances its activity. Luciferase bound to tetraiodofluorescien-Sepharose via a metal ion can subsequently be eluted with appropriate ligands. Luciferase was found to be sensitive to the spacer geometry of immobilized tetraiodofluorescein.
根据动力学失活研究，四碘荧光素以明显竞争的方式与荧光素酶结合。在所研究的金属离子中，Cu2+和Ni2+是第一过渡系中仅有的两种能增强染料对酶失活作用的离子。Mg2+、Co2+和Mn2+似乎具有保护作用，而Zn2+则可防止酶失活，甚至增强其活性。通过金属离子与四碘荧光素-琼脂糖结合的荧光素酶随后可用适当的配体洗脱。发现荧光素酶对固定化四碘荧光素的间隔基几何形状敏感。
