转化细胞中的丝状肌动蛋白聚集体。
F-actin aggregates in transformed cells.
作者信息
Carley W W, Barak L S, Webb W W
出版信息
J Cell Biol. 1981 Sep;90(3):797-802. doi: 10.1083/jcb.90.3.797.
Abstract
Polymerized actin has been found aggregated into distinctive patches inside transformed cells in culture. The F-actin-specific fluorescent probe, nitrobenzoxadiazole-phallacidin, labels these F-actin aggregates near the ventral cell surface of cells transformed by RNA or DNA tumor viruses, or by chemical mutagens, or spontaneously. Their appearance in all eight transformed cell types studied suggests their ubiquity and involvement in transformation morphology. Actin patches developed in normal rat kidney (NRK) cells transformed by a temperature-sensitive mutant of Rous sarcoma virus (LA23-NRK) within 30 min after a shift from the nonpermissive (39 degrees C) to the permissive temperature (32 degrees C). Patch appearance paralleling viral src gene expression tends to implicate pp60src kinase activity in destabilizing the cytoskeleton. However, appearance of the actin aggregates in cells not transformed by retrovirus calls for alternative mechanisms, perhaps involving an endogenous kinase, for this apparently common trait.
摘要
已发现聚合肌动蛋白在培养的转化细胞内聚集成独特的斑块。F-肌动蛋白特异性荧光探针，即硝基苯并恶二唑-鬼笔环肽，可标记这些F-肌动蛋白聚集体，它们位于经RNA或DNA肿瘤病毒、化学诱变剂转化或自发转化的细胞的腹侧细胞表面附近。在研究的所有八种转化细胞类型中它们的出现表明其普遍性以及与转化形态有关。在从非允许温度（39℃）转变为允许温度（32℃）后30分钟内，由劳氏肉瘤病毒的温度敏感突变体（LA23-NRK）转化的正常大鼠肾（NRK）细胞中形成了肌动蛋白斑块。斑块的出现与病毒src基因表达平行，这倾向于表明pp60src激酶活性在破坏细胞骨架稳定性中起作用。然而，在未被逆转录病毒转化的细胞中肌动蛋白聚集体的出现需要其他机制，可能涉及一种内源性激酶，来解释这一明显的共同特征。
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