Wickus G, Gruenstein E, Robbins P W, Rich A
Proc Natl Acad Sci U S A. 1975 Feb;72(2):746-9. doi: 10.1073/pnas.72.2.746.
The actin content of membranes prepared from cultured chick embryo fibroblasts has been measured on polyacrylamide gels. The actin was identified by tryptic peptide mapping. After transformation of the cells by Rous sarcoma virus, the amount of actin associated with the membranes is decreased by 30-50%. This result is not due to infection per se, since infection by a temperature-sensitive strain of the virus decreases membrane-associated actin only at the permissive temperature. A shift from the nonpermissive (41 degrees) to the permissive (36 degrees) temperature results in an increase in the percentage of total cellular protein synthesis devoted to actin production, so that the decrease in membrane-associated actin appears to be a selective displacement from the membrane rather than a general decrease in total cellular actin.
已在聚丙烯酰胺凝胶上测定了从培养的鸡胚成纤维细胞制备的膜中的肌动蛋白含量。通过胰蛋白酶肽图谱鉴定肌动蛋白。用劳斯肉瘤病毒转化细胞后，与膜相关的肌动蛋白量减少了30%-50%。该结果并非源于感染本身，因为用病毒的温度敏感株感染仅在允许温度下才会降低膜相关肌动蛋白。从非允许温度（41摄氏度）转变为允许温度（36摄氏度）会导致用于肌动蛋白产生的总细胞蛋白质合成百分比增加，因此膜相关肌动蛋白的减少似乎是从膜上的选择性转移，而非细胞总肌动蛋白的普遍减少。
