Mazurenko N P, Merekalova Z I, Pavlish O A, Bykovsky A F, Kurzman M J, Mazurenko N N
Neoplasma. 1981;28(4):403-12.
The results of studies of a co-carcinogenic effect of two human infectious viruses in tissue culture are reported here. Viable vaccinia virus actively replicating in the cells of primary BALB/c tissue culture and in a number of continuous murine cell lines has been shown to induce in them expression of major structural p30 protein of murine retroviruses. Vaccinia virus has been also shown to cause biochemical transformation of murine cells. Evidence for the capacity of herpes simplex virus type 2 to induce malignant transformation of BALB/3T3 murine cell line has been obtained and confirmed by transplantation to mice. Transformed cell clones did not contain complete infectious herpes simplex virus but were resistant to superinfection with this virus. N-tropic endogenous murine retrovirus of C type with buoyant density in the saccharose density gradient of 1.18 g/cm3 and a reverse transcriptase activity was expressed in the transformed cells. In the virion structure six proteins typical of these viruses with the prevalence of p30 have been demonstrated. Competitive radioimmunoassay revealed a very high level of virus production: p30 level reached 7500 ng p30 R-MuLV per mg of viral protein. Specificity of this results was shown in control experiments.
本文报道了两种人类感染性病毒在组织培养中的促癌作用研究结果。在原代BALB/c组织培养细胞和一些连续的小鼠细胞系中活跃复制的活痘苗病毒已被证明能诱导它们表达小鼠逆转录病毒的主要结构p30蛋白。痘苗病毒也已被证明能引起小鼠细胞的生化转化。已获得单纯疱疹病毒2型诱导BALB/3T3小鼠细胞系恶性转化的证据，并通过移植到小鼠体内得到证实。转化细胞克隆不含完整的感染性单纯疱疹病毒，但对该病毒的超感染具有抗性。在转化细胞中表达了C型N-嗜性内源性小鼠逆转录病毒，其在蔗糖密度梯度中的浮力密度为1.18 g/cm3，具有逆转录酶活性。在病毒粒子结构中，已证明有六种典型的此类病毒蛋白，其中p30占优势。竞争性放射免疫分析显示病毒产生水平非常高：每毫克病毒蛋白中p30水平达到7500 ng p30 R-MuLV。对照实验表明了该结果的特异性。
