Carter P
Clin Chem. 1978 Feb;24(2):362-4.
I describe a convenient method for stripping hormones and other ligands from human serum. Formation of a serum/charcoal colloid is avoided by incubating serum on a limited surface area with activated charcoal, in a lateral oscillating shaker. Particle-free serum of negligible turbidity is obtained without ultracentrifugation by ejecting the centrifugate through a cellulose ester membrane. By this technique, more than 80% of thyrotropin, triiodothyronine, thyroxine, cortisol, insulin, digoxin, folate, estriol, or an ether derivative of testosterone is removed from serum. About 70% of follitropin and somatotropin are removed, as well as half the choriogonadotropin. Serum protein remains intact throughout the adsorption process.
我描述了一种从人血清中去除激素和其他配体的简便方法。通过在侧向振荡摇床上，将血清在有限表面积上与活性炭孵育，避免了血清/活性炭胶体的形成。通过将离心液通过纤维素酯膜喷出，无需超速离心即可获得浊度可忽略不计的无颗粒血清。通过这种技术，超过80%的促甲状腺激素、三碘甲状腺原氨酸、甲状腺素、皮质醇、胰岛素、地高辛、叶酸、雌三醇或睾酮的醚衍生物从血清中被去除。约70%的促卵泡激素和生长激素以及一半的绒毛膜促性腺激素被去除。血清蛋白在整个吸附过程中保持完整。
