Bidey S P, Marshall N J, Ekins R P
Acta Endocrinol (Copenh). 1981 Nov;98(3):370-6. doi: 10.1530/acta.0.0980370.
The cyclic AMP response to thyrotrophin (TSH) has been investigated in cells prepared from human thyroid tissue obtained during surgery for subtotal laryngectomy, and maintained under in vitro conditions as primary monolayer cultures. When cells were incubated with 1.0 mU TSH/ml, a maximal level of intracellular cyclic AMP was reached after 20 min of incubation in the presence of 0.5 mM 3-isobutyl-1-methyl xanthine (MIX). This level of cyclic AMP was sustained for at least 2 h. Half-maximal stimulation of cyclic AMP was produced by TSH doses of between 1 and 5 mU/ml. In a study of a series of eight groups of monolayer cultures, each derived from a single, different thyroid gland, the mean stimulation of cyclic AMP given by 50 mU TSH/ml was 37.8-fold greater than in non-stimulated cell monolayers. Significant stimulation of 50 microunits TSH/ml was observed in some monolayers and the precision of measurement of TSH was better than 15% over the TSH dose range 0.2-1.0 mU/ml. The magnitude of the cyclic AMP response to TSH was unaffected by the presence in the incubation medium of 20% (v/v) normal human serum. A cyclic AMP response to TSH was still demonstrable in cells that had been maintained for a period of 22 days in monolayer culture, although the response was reduced in comparison with that given by 4-5 day old cultures.
在因次全喉切除术而在手术中获取的人甲状腺组织制备的细胞中，研究了促甲状腺激素（TSH）对环磷酸腺苷（cAMP）的反应，并将这些细胞作为原代单层培养物在体外条件下进行培养。当细胞与1.0 mU TSH/ml一起孵育时，在存在0.5 mM 3 -异丁基-1 -甲基黄嘌呤（MIX）的情况下孵育20分钟后，细胞内cAMP达到最大水平。该cAMP水平至少维持2小时。TSH剂量在1至5 mU/ml之间时可产生半最大刺激的cAMP。在一项对八组单层培养物的研究中，每组均来自单个不同的甲状腺，50 mU TSH/ml所产生的cAMP平均刺激比未刺激的细胞单层高37.8倍。在一些单层培养物中观察到50微单位TSH/ml有显著刺激，并且在0.2 - 1.0 mU/ml的TSH剂量范围内，TSH测量精度优于15%。孵育培养基中存在20%（v/v）正常人血清对TSH引起的cAMP反应幅度没有影响。在单层培养中维持了22天的细胞中，对TSH的cAMP反应仍然可以检测到，尽管与4 - 5日龄培养物相比，反应有所降低。
