Bauknecht T, Krahe B, Rechenbach U, Zahradnik H P, Breckwoldt M
Acta Endocrinol (Copenh). 1981 Nov;98(3):446-50. doi: 10.1530/acta.0.0980446.
Human myometrium was studied for specific binding of PGE2 and PGF2 alpha. PGF2 alpha-binding was almost undetectable, but specific binding sites for PGE2 with high affinity (KD = 2.7 +/- 0.4 x 10(-9) M were demonstrated. The binding capacity for PGE2 exhibited a topically different distribution pattern with the highest values in the central parts and low to undetectable levels in the cervical region. Binding characteristics were analyzed by receptor kinetics, revealing a homogeneous receptor population. Binding capacity in uteri obtained from post-menopausal women was of the order of 900-940 fmol/mg protein. Oestrogen pre-treatment and pregnancy were associated with a 3-fold reduction of the PGE2-binding capacity.
对人子宫肌层进行了前列腺素E2（PGE2）和前列腺素F2α（PGF2α）特异性结合的研究。几乎检测不到PGF2α结合，但证明了存在对PGE2具有高亲和力的特异性结合位点（解离常数KD = 2.7 +/- 0.4 x 10(-9) M）。PGE2的结合能力呈现出局部不同的分布模式，中部的值最高，宫颈区域的值低至无法检测到。通过受体动力学分析结合特性，揭示了受体群体的均一性。绝经后妇女子宫的结合能力约为900 - 940 fmol/mg蛋白质。雌激素预处理和妊娠与PGE2结合能力降低3倍有关。
