Joh K, Riede U N, Zahradnik H P
Department of Pathology, University of Freiburg, F.R.Germany.
J Exp Pathol (Oxford). 1990 Apr;71(2):171-86.
The aim of this study was to investigate the effect of prostaglandins (PGs) on the release of lysosomes into the extracellular space in experimentally-induced storage disease in rats. The generalized phospholipidosis was induced by oral administration of chlorphentermine to Wistar rats. PG-E2 or PG-F2 alpha (50 micrograms/100 g) was injected intravenously into rats with phospholipidosis. Control rats were injected with the same amount of saline. Twenty-four hours later, extracellular and intracellular lysosomes were analysed morphometrically in electromicrographs of the liver, kidney, myocardium, skeletal muscle, aorta and cervix uteri, having been stained with acid-phosphatase. The morphometric parameters used were the numerical density of lysosomes per unit volume and the volume density of lysosomes per unit volume. The results show that, in comparison with the controls, a volumetric increase of extracellular lysosomes and a volumetric decrease of intracellular lysosomes were achieved by PG-E2 or PG-F2 alpha administration in several organs, but not in the myocardium or skeletal muscles. The effect of PG-F2 alpha was greater than that of PG-E2 in the liver and kidney, while the effect of PG-E2 was greater than that of PG-F2 alpha in the aorta and cervix uteri. These results indicate that PGs influence the discharge of storage lysosomes in bipolar epithelial cells in the liver and kidney, and also influence the release of non-storing lysosomes in apolar mesenchymal cells in the aorta and cervix uteri.
本研究的目的是探讨前列腺素（PGs）对实验性诱导的大鼠贮积病中溶酶体释放到细胞外空间的影响。通过向Wistar大鼠口服氯苯丁胺诱导全身性磷脂沉积症。将PG-E2或PG-F2α（50微克/100克）静脉注射到患有磷脂沉积症的大鼠体内。对照大鼠注射等量的生理盐水。24小时后，在肝脏、肾脏、心肌、骨骼肌、主动脉和子宫颈的电子显微镜照片中，用酸性磷酸酶染色后对细胞外和细胞内溶酶体进行形态计量分析。所使用的形态计量参数是每单位体积溶酶体的数量密度和每单位体积溶酶体的体积密度。结果表明，与对照组相比，在几个器官中，通过给予PG-E2或PG-F2α可使细胞外溶酶体体积增加，细胞内溶酶体体积减少，但在心肌或骨骼肌中未出现这种情况。在肝脏和肾脏中，PG-F2α的作用大于PG-E2，而在主动脉和子宫颈中，PG-E2的作用大于PG-F2α。这些结果表明，PGs影响肝脏和肾脏中双极上皮细胞中贮存溶酶体的排出，也影响主动脉和子宫颈中非贮存溶酶体在无极性间充质细胞中的释放。
