亚硝酸盐与人类血红蛋白相互作用过程中超氧化物和过氧化氢的生成。

Generation of superoxide and hydrogen peroxide during interaction of nitrite with human hemoglobin.

作者信息

Watanabe S, Ogata M

出版信息

Acta Med Okayama. 1981 Jun;35(3):173-8. doi: 10.18926/AMO/31285.

Abstract

Generation of superoxide and hydrogen peroxide during interaction of nitrite with human hemoglobin was detected by chemiluminescence of luminol. Luminol chemiluminescence was inhibited by the addition of superoxide dismutase (SOD) and catalase. Methemoglobin formation induced by nitrite was also inhibited by the addition of SOD and catalase. The mechanism of methemoglobin formation by nitrite was discussed in regard to the oxidation of hemoglobin by superoxide and hydrogen peroxide as generated by the interaction of nitrite with hemoglobin.

摘要

通过鲁米诺化学发光法检测了亚硝酸盐与人血红蛋白相互作用过程中超氧化物和过氧化氢的生成。超氧化物歧化酶（SOD）和过氧化氢酶的添加抑制了鲁米诺化学发光。亚硝酸盐诱导的高铁血红蛋白形成也受到SOD和过氧化氢酶添加的抑制。关于亚硝酸盐与血红蛋白相互作用产生的超氧化物和过氧化氢对血红蛋白的氧化作用，讨论了亚硝酸盐形成高铁血红蛋白的机制。