Schwartz S A
Biosci Rep. 1981 Feb;1(2):107-18. doi: 10.1007/BF01117007.
Control and bromodeoxyuridine-containing rat-embryo-cell DNA were digested by the restriction endonucleases Hpa II and Msp I and were subsequently analyzed by agarose-gel electrophoresis as well as DNA-affinity chromatography. By the former technique, it appeared that no substantial differences existed between the two DNA samples with respect to the amount or distribution of methylcytosine. On the other hand, it was obvious following base-specific DNA chromatography that the virogenic analog was markedly concentrated in particular nucleotide sequences which demonstrated a proportionately greater affinity for the (A-T)-specific adsorbent irrespective of digestion by either restriction endonuclease.
用限制性内切酶Hpa II和Msp I消化对照和含溴脱氧尿苷的大鼠胚胎细胞DNA，随后通过琼脂糖凝胶电泳以及DNA亲和色谱法进行分析。通过前一种技术，似乎两个DNA样品在甲基胞嘧啶的量或分布方面没有实质性差异。另一方面，在碱基特异性DNA色谱分析后很明显，病毒原性类似物明显集中在特定的核苷酸序列中，无论用哪种限制性内切酶消化，这些序列对（A-T）特异性吸附剂都表现出相应更大的亲和力。
