Franz T, Waehneldt T V, Neuhoff V, Wächtler K
Brain Res. 1981 Dec 7;226(1-2):245-58. doi: 10.1016/0006-8993(81)91097-0.
CNS myelin was isolated by a conventional method from a wide range of vertebrate classes and analyzed by SDS-PAGE for proteins (Coomassie blue) and glycoproteins (concanavalin A (Con-A)-peroxidase). Mammalian, avian and reptilian myelin shared similar protein patterns (basic protein, BP; intermediate protein, DM-20; proteolipid protein, PLP; Wolfgram protein, W). Amphibians lacked DM-20 but were characterized by specific activities of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) higher than those of the other classes examined. The Con A-binding profiles were similar in the high molecular weight (HMW) regions of the classes listed above, while the typical myelin proteins in the low molecular weight (LMW) regions were devoid of Con A-binding properties. In teleost myelin a putative BP band ran well ahead of rat small basic protein (SBP), whereas the region corresponding to rat PLP was covered by several closely spaced bands, most of which bound Con A. In elasmobranch myelin, apart from bands corresponding to BP, Con A-binding glycoproteins were detected migrating in the region of rat DM-20 and PLP as well as with mammalian PNS P0 protein. Cyclostomates yielded only very small amounts of material in the myelin preparation and displayed undifferentiated Coomassie blue- and Con A-binding in the HMW region, while typical LMW myelin proteins were absent. These results demonstrate that CNS myelin from bony and cartilaginous fishes is characterized by containing several major Con A-binding proteins of low molecular weight. This is in striking contrast to myelin from phylogenetically higher classes.
采用传统方法从多种脊椎动物类别中分离出中枢神经系统髓磷脂，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析其蛋白质（考马斯亮蓝染色）和糖蛋白（伴刀豆球蛋白A（Con-A）-过氧化物酶染色）。哺乳动物、鸟类和爬行动物的髓磷脂具有相似的蛋白质模式（碱性蛋白，BP；中间蛋白，DM-20；蛋白脂蛋白，PLP；沃尔夫格拉姆蛋白，W）。两栖动物缺乏DM-20，但2',3'-环核苷酸3'-磷酸二酯酶（CNP）的比活性高于所检测的其他类别。上述类别在高分子量（HMW）区域的Con A结合图谱相似，而低分子量（LMW）区域的典型髓磷脂蛋白缺乏Con A结合特性。在硬骨鱼髓磷脂中，一条假定的BP带迁移速度比大鼠小碱性蛋白（SBP）快得多，而对应于大鼠PLP的区域被几条紧密间隔的条带覆盖，其中大部分与Con A结合。在板鳃亚纲动物髓磷脂中，除了对应于BP的条带外，还检测到Con A结合糖蛋白在大鼠DM-20和PLP区域以及与哺乳动物外周神经系统P0蛋白一起迁移。圆口纲动物在髓磷脂制备中仅产生极少量物质，在HMW区域显示未分化的考马斯亮蓝染色和Con A结合，而缺乏典型的LMW髓磷脂蛋白。这些结果表明，硬骨鱼和软骨鱼的中枢神经系统髓磷脂的特征是含有几种低分子量的主要Con A结合蛋白。这与系统发育上较高类别的髓磷脂形成鲜明对比。
