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人类和小鼠的髓鞘蛋白质组和少突胶质细胞转录组图谱的保守性和差异性。

Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.

机构信息

Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.

Neuroproteomics Group, Department of Molecular Neurobiology, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.

出版信息

Elife. 2022 May 11;11:e77019. doi: 10.7554/eLife.77019.

DOI:10.7554/eLife.77019
PMID:35543322
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9094742/
Abstract

Human myelin disorders are commonly studied in mouse models. Since both clades evolutionarily diverged approximately 85 million years ago, it is critical to know to what extent the myelin protein composition has remained similar. Here, we use quantitative proteomics to analyze myelin purified from human white matter and find that the relative abundance of the structural myelin proteins PLP, MBP, CNP, and SEPTIN8 correlates well with that in C57Bl/6N mice. Conversely, multiple other proteins were identified exclusively or predominantly in human or mouse myelin. This is exemplified by peripheral myelin protein 2 (PMP2), which was specific to human central nervous system myelin, while tetraspanin-2 (TSPAN2) and connexin-29 (CX29/GJC3) were confined to mouse myelin. Assessing published scRNA-seq-datasets, human and mouse oligodendrocytes display well-correlating transcriptome profiles but divergent expression of distinct genes, including and . A searchable web interface is accessible via www.mpinat.mpg.de/myelin. Species-dependent diversity of oligodendroglial mRNA expression and myelin protein composition can be informative when translating from mouse models to humans.

摘要

人类髓鞘疾病通常在小鼠模型中进行研究。由于两个进化枝大约在 8500 万年前就已经分化，因此了解髓鞘蛋白组成在多大程度上保持相似至关重要。在这里，我们使用定量蛋白质组学分析从人白质中纯化的髓鞘，发现髓鞘碱性蛋白、髓鞘少突胶质细胞糖蛋白、髓鞘结合蛋白、少突胶质细胞成熟相关蛋白 8 的相对丰度与人 C57Bl/6N 小鼠的髓鞘中的相对丰度密切相关。相反，许多其他蛋白质仅在人类或小鼠的髓鞘中被鉴定为特异性存在或主要存在。例如，外周髓鞘蛋白 2（PMP2）特异性存在于人类中枢神经系统髓鞘中，而四跨膜蛋白 2（TSPAN2）和连接蛋白 29（CX29/GJC3）则局限于小鼠的髓鞘中。评估已发表的 scRNA-seq 数据集，人类和小鼠少突胶质细胞显示出良好相关的转录组图谱，但不同基因的表达存在差异，包括 和 。通过 www.mpinat.mpg.de/myelin 可以访问一个可搜索的网络界面。当从小鼠模型转化到人类时，少突胶质细胞 mRNA 表达和髓鞘蛋白组成的物种依赖性多样性具有重要的信息价值。

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FEBS J. 2021 Dec;288(23):6716-6735. doi: 10.1111/febs.16079. Epub 2021 Jul 14.
3
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