Maeshima M, Asahi T
J Biochem. 1981 Aug;90(2):399-406. doi: 10.1093/oxfordjournals.jbchem.a133486.
A protein, which was immunoreactive to antibody against cytochrome c oxidase, was found in the mitochondrial membrane fraction of sweet potato root tissue. The protein was associated relatively weakly with the mitochondrial inner membrane as compared with cytochrome c oxidase. It exerted no cytochrome c oxidase activity and contained no heme a. The protein was purified by phenyl-Sepharose column chromatography and polyacrylamide gel electrophoresis. The molecular weight of its polypeptide chain was 57,000. In addition, the protein decreased during aging of tissue slices. It is therefore not improbable that the protein is a precursor of cytochrome c oxidase composed of only the subunits of cytoplasmic origin, since aging of tissue slices has been shown to result in an increase in the enzyme activity which is inhibited by chloramphenicol but not by cycloheximide.
在甘薯根组织的线粒体膜组分中发现了一种与抗细胞色素c氧化酶抗体发生免疫反应的蛋白质。与细胞色素c氧化酶相比，该蛋白质与线粒体内膜的结合相对较弱。它不具有细胞色素c氧化酶活性，也不含血红素a。该蛋白质通过苯基琼脂糖柱层析和聚丙烯酰胺凝胶电泳进行纯化。其多肽链的分子量为57,000。此外，在组织切片老化过程中该蛋白质含量下降。因此，该蛋白质不太可能是仅由细胞质来源的亚基组成的细胞色素c氧化酶的前体，因为已表明组织切片老化会导致酶活性增加，而氯霉素可抑制该酶活性，环己酰亚胺则不能。
