Matsuoka M, Maeshima M, Asahi T
J Biochem. 1981 Sep;90(3):649-55. doi: 10.1093/oxfordjournals.jbchem.a133519.
Cytochrome c oxidase was purified from pea shoots in a form containing more than 12 nmol of heme a per mg protein, but rapid inactivation took place during purification. On slab polyacrylamide concentration gradient gel electrophoresis of a partially purified preparation, there were three activity-bands corresponding to main protein bands. The activity-bands, as well as the most purified preparation, contained five polypeptides of different molecular weights (39,000, 33,000, 28,500, 16,500, and 8,000-6,000) as shown by sodium dodecylsulfate-urea polyacrylamide gel electrophoresis. However, an immunoprecipitate from the partially purified preparation with antibody against the most purified preparation contained two additional polypeptides with molecular weights of 13,000 and 10,000. Pea cytochrome c oxidase resembled the sweet potato enzyme with respect to immunological properties and absorption spectra as well as the subunit composition. We propose that higher plant cytochrome c oxidase is composed of five subunits of different molecular weights and is associated weakly with two low-molecular-weight polypeptides in the mitochondrial inner membrane.
细胞色素c氧化酶是从豌豆嫩枝中纯化得到的，其形式为每毫克蛋白质含有超过12纳摩尔的血红素a，但在纯化过程中会迅速失活。在部分纯化制剂的平板聚丙烯酰胺浓度梯度凝胶电泳中，有三条与主要蛋白带相对应的活性带。如十二烷基硫酸钠-尿素聚丙烯酰胺凝胶电泳所示，这些活性带以及最纯的制剂含有五种不同分子量的多肽（39000、33000、28500、16500和8000 - 6000）。然而，用针对最纯制剂的抗体对部分纯化制剂进行免疫沉淀得到的沉淀物含有另外两种分子量分别为13000和10000的多肽。豌豆细胞色素c氧化酶在免疫特性、吸收光谱以及亚基组成方面与甘薯酶相似。我们提出高等植物细胞色素c氧化酶由五个不同分子量的亚基组成，并且在线粒体内膜中与两种低分子量多肽弱相关。
