人乳头多瘤空泡病毒BK感染性DNA在大肠杆菌中的分子克隆
Molecular cloning of infectious DNA from human papovavirus BK in Escherichia coli.
作者信息
Akrigg A, Gardner S D, Greenaway P J
出版信息
J Gen Virol. 1981 Jul;55(Pt 1):247-52. doi: 10.1099/0022-1317-55-1-247.
PMID:6271906
Abstract
Recombinant DNA constructed from unit length BK virus DNA and from several defective viral forms was cloned in Escherichia coli HB101. The cloned unit length BK virus DNA retained its infectivity for human embryonic kidney cells, whereas the cloned defective DNA showed no infectivity. Restriction endonuclease digestion of cloned defective DNA and purified virus DNA indicated that many of the defective forms contain re-iterated sequences.
摘要
由单位长度的BK病毒DNA和几种缺陷病毒形式构建的重组DNA被克隆到大肠杆菌HB101中。克隆的单位长度BK病毒DNA对人胚肾细胞仍具有感染性，而克隆的缺陷DNA则无感染性。对克隆的缺陷DNA和纯化的病毒DNA进行限制性内切酶消化表明，许多缺陷形式含有重复序列。
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