培养的大鼠肌管中反常整流的单钾离子通道电流
Single K+ channel currents of anomalous rectification in cultured rat myotubes.
作者信息
Ohmori H, Yoshida S, Hagiwara S
出版信息
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4960-4. doi: 10.1073/pnas.78.8.4960.
Abstract
The currents through single K+ channels of the anomalous (or inward) rectifier were recorded in tissue cultured rat myotubes by using the "gigohm seal" patch clamp technique developed by Sigworth and Neher. These unitary currents were detected as current fluctuations due to the blocking and unblocking of channels by Ba2+. The single-channel conductance was obtained from the slope of the linear relationship between unitary current amplitude and membrane potential. When the external solution contained 155 mM K+, the single-channel conductance was 10.4 +/- 2.6 pS (+/- SD; n = 6). This value was independent of the the concentration of blocking ions but increased with increasing external K+ concentration. The behavior of the unitary current agreed with that expected from the blocking kinetics of Ba2+ on the macroscopic K+ current of the anomalous rectifier. The density of the channel is likely to be small and may even be less than 1/micrometers 2.
摘要
运用西格沃思和内尔开发的“千兆欧封接”膜片钳技术，在组织培养的大鼠肌管中记录了反常（或内向）整流器单个钾离子通道的电流。由于钡离子对通道的阻断和解除阻断作用，这些单位电流被检测为电流波动。单通道电导由单位电流幅度与膜电位之间线性关系的斜率得出。当外部溶液含有155 mM钾离子时，单通道电导为10.4±2.6 pS（±标准差；n = 6）。该值与阻断离子的浓度无关，但随外部钾离子浓度的增加而增加。单位电流的行为与钡离子对反常整流器宏观钾电流的阻断动力学预期相符。通道密度可能较小，甚至可能小于1/平方微米。
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