Jones D P
Res Commun Chem Pathol Pharmacol. 1981 Aug;33(2):215-22.
The effect of preincubation with alpha-methyldopa on catalase-dependent decomposition of H2O2 was studied in isolated rat hepatocytes. A direct assay of catalase function was obtained by measurement of O2 production under anaerobic conditions following additions of H2O2. Both the total O2 produced and the maximal O2 concentration attained were decreased by preincubation with alpha-methyldopa. The results demonstrate that alpha-methyldopa-induced formation of Compound II is associated with loss of intracellular catalase activity. These results indicate that the hepatotoxicity of alpha-methyldopa may be related to an increased sensitivity to oxidative injury due to inhibited catalase function.
在分离的大鼠肝细胞中研究了用α-甲基多巴预孵育对过氧化氢酶依赖性过氧化氢分解的影响。通过在添加过氧化氢后在厌氧条件下测量氧气产生来直接测定过氧化氢酶功能。用α-甲基多巴预孵育会降低产生的总氧量和达到的最大氧浓度。结果表明，α-甲基多巴诱导的化合物II的形成与细胞内过氧化氢酶活性的丧失有关。这些结果表明，α-甲基多巴的肝毒性可能与由于过氧化氢酶功能受抑制而对氧化损伤的敏感性增加有关。
