Staník J, Tetrevová J, Izariková A, Cierna V
Vet Med (Praha). 1981 Sep;26(9):517-23.
Nine dairy cows aged four to eleven years were subjected to examination by chromosomal analysis. These cows had been found, by haematological examination, to suffer from leucosis. The study also covered one ten-day-old calf - heifer. The test group included two dam-daughter pairs. The animals belonged to the Black-Pied Lowland breed. The blood was sampled from vena jugularis and the karyotypes were processed and evaluated by the method after Moorhead et al. (1960), modified by Lojda et al. (1974). A list was kept for each animal. The tested animals were included in classes by the percentages of the chromosome aberrations: class I - two animals (up to 10% of aberrations), class II - seven animals (from 10% to 20% of aberrations), class III - one animal (above 20% of aberrations). Hyposomy was found in all cases, polysomy and hyperploidy in four cases. Structural aberrations were observed in nine cases, breaks being the most frequent anomalies (7 cases). Breaks on sexual chromosomes were observed in five cases, including the dam-daughter pairs; centric fusion occurred in one case and mixed aberrations in two cases.
对9头年龄在4至11岁的奶牛进行了染色体分析检查。通过血液学检查发现这些奶牛患有白血病。该研究还涵盖了一头10日龄的小母牛。试验组包括两对母女。这些动物属于黑花低地品种。从颈静脉采集血液,按照Moorhead等人(1960年)的方法进行处理并评估核型,该方法由Lojda等人(1974年)修改。为每只动物建立了一份清单。根据染色体畸变的百分比将受试动物分为不同类别:I类 - 2只动物(畸变率高达10%),II类 - 7只动物(畸变率为10%至20%),III类 - 1只动物(畸变率高于20%)。在所有病例中均发现了亚二倍体,4例出现了多体性和超倍体。观察到9例结构畸变,断裂是最常见的异常情况(7例)。在5例中观察到性染色体断裂,包括母女对;1例发生了着丝粒融合,2例出现了混合畸变。