Reinhardt T A, Napoli J L, Praminik B, Littledike E T, Beitz D C, Partridge J J, Uskoković M R, Horst R L
Biochemistry. 1981 Oct 13;20(21):6230-5. doi: 10.1021/bi00524a050.
A new metabolite of vitamin D3 has been isolated from the plasma of vitamin D3 treated cows and has been generated from 25(S),26-dihydroxyvitamin D3 with homogenates of vitamin D deficient chick kidney. This metabolite has been identified as 1,25,26-trihydroxyvitamin D3 by comigration with synthetic 1,25(S),26-trihydroxyvitamin D3 in four chromatographic systems, ultraviolet spectroscopy, mass spectrometry, and high-pressure liquid chromatography and mass spectrometry of derivatives. 1,25(S),26-Trihydroxyvitamin D3 is one-tenth as effective as 1,25-dihydroxyvitamin D3 in binding to the chick intestinal cytosol 1,25-dihydroxyvitamin D receptor. Either 25(S),26-dihydroxyvitamin D3 or 1,25-dihydroxyvitamin D3 can serve as precursor for in vitro production of 1,25,26-trihydroxyvitamin D3 by chick kidney tissue.
一种新的维生素D3代谢产物已从经维生素D3处理的奶牛血浆中分离出来，并且可通过用维生素D缺乏的雏鸡肾脏匀浆处理25(S),26 - 二羟基维生素D3而生成。通过在四个色谱系统中与合成的1,25(S),26 - 三羟基维生素D3共迁移、紫外光谱法、质谱法以及衍生物的高压液相色谱和质谱法，已将该代谢产物鉴定为1,25,26 - 三羟基维生素D3。1,25(S),26 - 三羟基维生素D3与雏鸡肠细胞溶质1,25 - 二羟基维生素D受体结合的效力仅为1,25 - 二羟基维生素D3的十分之一。25(S),26 - 二羟基维生素D3或1,25 - 二羟基维生素D3均可作为雏鸡肾脏组织体外生成1,25,26 - 三羟基维生素D3的前体。
