过碘酸盐诱导的人外周血淋巴细胞转化以及由此导致的膜唾液酸、半乳糖和岩藻糖残基的氧化。

Periodate-induced transformation of human peripheral blood lymphocytes and the resultant oxidation of membrane sialyl, galactosyl and fucosyl residues.

作者信息

Banchereau J F, Danois D M, Guenounou M, Durand G M, Agneray J C

出版信息

Biochim Biophys Acta. 1981 Nov 18;678(1):98-105. doi: 10.1016/0304-4165(81)90052-0.

Abstract

Oxidation of human peripheral mononuclear cells with sodium periodate results in lymphocyte activation. Period-date, at optimal mitogenic concentrations, oxidizes membrane sialyl residues (NeuNAc) essentially into the 7 carbon analogue (C7-NeuNAc). Fucosyl and galactosyl residues are also oxidized by periodate, since propane 1,2-diol and glycerol are isolated in acid hydrolysates of lymphocytes oxidized by periodate and reduced by tritiated borohydride. The neuraminidase pretreatment of lymphocytes induces a 40-50% decrease of their response to periodate. Neuraminidase treatment of 108 human peripheral lymphocytes liberated 9.6 microgram NeuNAc (31 nmol), representing 68.5% of the total content. The neuraminidase treatment dramatically enhances the recovery of glycerol in hydrolysates of lymphocytes treated successively with periodate and tritiated borohydride.

摘要

用高碘酸钠氧化人外周血单个核细胞会导致淋巴细胞活化。在最佳促有丝分裂浓度下，高碘酸盐将膜唾液酸残基（NeuNAc）基本上氧化成7碳类似物（C7-NeuNAc）。由于在经高碘酸盐氧化并用氚化硼氢化钠还原的淋巴细胞的酸水解物中分离出了1,2-丙二醇和甘油，岩藻糖基和半乳糖基残基也会被高碘酸盐氧化。淋巴细胞经神经氨酸酶预处理后，其对高碘酸盐的反应降低40 - 50%。用神经氨酸酶处理108个人外周血淋巴细胞可释放出9.6微克NeuNAc（31纳摩尔），占总含量的68.5%。神经氨酸酶处理显著提高了先后经高碘酸盐和氚化硼氢化钠处理的淋巴细胞水解物中甘油的回收率。