通过神经氨酸酶和半乳糖氧化酶的序贯处理诱导淋巴细胞转化
Induction of lymphocyte transformation by sequential treatment with neuraminidase and galactose oxidase.
作者信息
Novogrodsky A, Katchalski E
出版信息
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1824-7. doi: 10.1073/pnas.70.6.1824.
Treatment of mouse-spleen cells with galactose oxidase (EC 1.1.3.9) after incubation with neuraminidase (EC 3.2.1.18) induced extensive blastogenesis. Treatment of the cells with galactose oxidase before incubation with neuraminidase had very little stimulatory effect. Either of these enzymes alone had practically no effect on the cells. The lymphocyte transformation induced by galactose oxidase in neuraminidase-treated cells was decreased upon reacting the cells with borohydride or hydroxylamine, whereas treatment with these compounds did not affect transformation of cells by concanavalin A. It is suggested that galactosyl residues exposed by the action of neuraminidase on the cell membrane are oxidized by galactose oxidase, and the aldehyde moiety thus formed is involved in the induction of blastogenesis.
用神经氨酸酶（EC 3.2.1.18）孵育小鼠脾细胞后，再用半乳糖氧化酶（EC 1.1.3.9）处理，可诱导广泛的细胞增殖。在与神经氨酸酶孵育前先用半乳糖氧化酶处理细胞，刺激作用很小。单独使用这两种酶中的任何一种对细胞几乎都没有影响。用硼氢化钠或羟胺处理经神经氨酸酶处理的细胞后，半乳糖氧化酶诱导的淋巴细胞转化作用减弱，而用这些化合物处理并不影响伴刀豆球蛋白A对细胞的转化作用。提示神经氨酸酶作用于细胞膜所暴露出的半乳糖基残基被半乳糖氧化酶氧化，由此形成的醛基部分参与了细胞增殖的诱导过程。
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