四甲铵对单钠通道电流的影响。
The effect of tetramethylammonium on single sodium channel currents.
作者信息
Horn R, Patlak J, Stevens C F
出版信息
Biophys J. 1981 Nov;36(2):321-7. doi: 10.1016/S0006-3495(81)84734-0.
Abstract
Voltage-dependent Na conductance of rat myotubes was studied by patch recordings of single-channels. The patches were excised from the cell with the patch electrode, and the cytoplasmic surface was bathed in either CsF or tetramethylammonium (TMA)-F. Inward currents were examined from -20 to -50 mV. In this range Cs and TMA both appeared to be nearly impermeant, but TMA blocked the channel in a voltage-dependent manner. A first-order blocking site was located a maximum of 89% of the way through the membrane field from the cytoplasmic surface.
摘要
通过单通道膜片钳记录研究了大鼠肌管的电压依赖性钠电导。用膜片电极从细胞上切除膜片，将胞质表面浸泡在氟化铯或四甲基铵（TMA）-F中。在-20至-50mV范围内检测内向电流。在此范围内，铯和TMA似乎几乎都不渗透，但TMA以电压依赖性方式阻断通道。一个一级阻断位点位于从胞质表面起穿过膜电场最多89%的位置。
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