具有复制特性的人工Tn2551转座子
[Artificial Tn2551 transposon with replicative properties].
作者信息
Khaĭkinson M Ia, Bogush V G, Stepanov A I
出版信息
Genetika. 1981;17(10):1762-70.
A hybrid plasmid, pBE10 was constructed. It consists of DNAs of RSF2124 (ColE1 :: Tn3) plasmid and pUB110 plasmid of Staphylococcus aureus. The latter can be stably maintained in Bacillus subtilis. BamHI cleaved pUB110 was introduced into the BamHI site of transposon Tn3 and the resulting enlarged Tn3 (Tn2551) was transposed from pBE10 onto phage lambda and than to pMB9 (Tc) and RSF1010(Sm Su) plasmids. Restriction and heteroduplex analysis of pMB9 :: Tn2551(pBE21) and RSF1010 :: :: TN2551(pBE32) was carried out. Plasmids pBE10, pBE21 and pBE32 demonstrated some kind of molecular instability when introduced by transformation into Bacillus subtilis.
构建了一种杂种质粒pBE10。它由RSF2124（ColE1::Tn3）质粒的DNA和金黄色葡萄球菌的pUB110质粒组成。后者能在枯草芽孢杆菌中稳定维持。用BamHI切割的pUB110被引入转座子Tn3的BamHI位点，产生的扩大的Tn3（Tn2551）从pBE10转座到噬菌体λ上，然后再转座到pMB9（Tc）和RSF1010（Sm Su）质粒上。对pMB9::Tn2551（pBE21）和RSF1010:::TN2551（pBE32）进行了限制性酶切和异源双链分析。当通过转化引入枯草芽孢杆菌时，质粒pBE10、pBE21和pBE32表现出某种分子不稳定性。
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