[各种质粒整合到枯草芽孢杆菌染色体中]
[Integration of various plasmids into the Bacillus subtilis chromosome].
作者信息
Khasanov F K, Bashkirov V I, Prozorov A A
出版信息
Genetika. 1985 Oct;21(10):1618-26.
Some features of integration of temperature-sensitive pE194, pGG10 and pGG20 plasmids into the Bacillus subtilis chromosome were studied. Several auxotrophic mutations were obtained using insertion of these plasmids into the chromosome. The sites of plasmids for illegitimate recombination were determined. It was shown that the integration into the Bac. subtilis chromosome is characteristic not only for the plasmid pE194 but is the property of Staphylococcus aureus plasmid pC194 and Escherichia coli pBR322 plasmid. The influence of different Bac. subtilis rec mutations on the frequency of integration was studied.
研究了温度敏感型pE194、pGG10和pGG20质粒整合到枯草芽孢杆菌染色体中的一些特征。通过将这些质粒插入染色体获得了几个营养缺陷型突变。确定了质粒进行异常重组的位点。结果表明，整合到枯草芽孢杆菌染色体中不仅是质粒pE194的特征，也是金黄色葡萄球菌质粒pC194和大肠杆菌pBR322质粒的特性。研究了不同枯草芽孢杆菌rec突变对整合频率的影响。
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