Lombardo Y B, Hron W T, Menahan L A
Diabetologia. 1978 Jan 14;14(1):47-51. doi: 10.1007/BF00429707.
Withdrawal of exogenous insulin and a subsequent fast (24 h) of alloxan diabetic rats stimulated rates of gluconeogenesis, urogenesis, ketogenesis, and amino acid release by in situ perfused livers when compared to those from normal, fasted rats. The contribution of liver glycogen to the high rates of gluconeogenesis observed with the diabetic liver could be excluded. Perfusate lactate concentrations remained constant during the period when the elevated rate of gluconeogenesis was observed with diabetic liver. Addition of insulin as a bolus (750 mU) and continuous infusion (12.5 mU/min) to the perfusion medium of diabetic livers resulted in constant perfusate levels of glucose, urea and alpha-amino nitrogen indicating a suppression of the catabolic processes present in the fasted, diabetic liver. The rate of ketogenesis was also slowed by insulin to about half the rate prior to addition of the hormone. These data indicate that insulin has an immediate anti-catabolic effect in the perfused, diabetic liver.
与正常禁食大鼠相比，撤除四氧嘧啶糖尿病大鼠的外源性胰岛素并随后禁食24小时，会刺激原位灌注肝脏的糖异生、尿素生成、酮体生成以及氨基酸释放速率。可以排除肝糖原对糖尿病肝脏中观察到的高糖异生速率的贡献。在观察到糖尿病肝脏糖异生速率升高的期间，灌注液中的乳酸浓度保持恒定。向糖尿病肝脏的灌注介质中一次性注射（750 mU）并持续输注（12.5 mU/分钟）胰岛素，会使灌注液中的葡萄糖、尿素和α-氨基氮水平保持恒定，这表明禁食的糖尿病肝脏中存在的分解代谢过程受到了抑制。胰岛素还使酮体生成速率减慢至添加该激素之前速率的约一半。这些数据表明，胰岛素对灌注的糖尿病肝脏具有即时的抗分解代谢作用。
