Nakamura T, Shimada K, Takagi Y
J Biochem. 1981 Oct;90(4):1013-22. doi: 10.1093/oxfordjournals.jbchem.a133553.
ColE1 derivatives carrying cohesive end sites of lambda phage genome (= cos lambda) can be packaged within lambda phage particles. The DNA structure of the prototype ColE1-cos lambda derivative named pKY2257 was studied because of its potential usefulness in various fields in molecular biology. pKY2257, which carries an intact galactose operon of E. coli, is a convenient replicon to detect Tn3 translocation. It was found that one of the PK2257::Tn3 derivatives, pKY2113, generated various small plasmids in E. coli. The molecular structures of some of these deletion mutants were compared with each other and with those of parental plasmid DNAs by heteroduplex analysis and restriction enzyme digestion. A possible mechanism, which seems to be unique to this kind of deletions, is discussed on the basis of the present results.
携带λ噬菌体基因组粘性末端位点（= cosλ）的ColE1衍生物可被包装到λ噬菌体颗粒中。由于其在分子生物学各个领域的潜在用途，对名为pKY2257的原型ColE1-cosλ衍生物的DNA结构进行了研究。pKY2257携带大肠杆菌完整的半乳糖操纵子，是检测Tn3易位的便利复制子。发现PK2257::Tn3衍生物之一pKY2113在大肠杆菌中产生了各种小质粒。通过异源双链分析和限制性内切酶消化，比较了其中一些缺失突变体与亲本质粒DNA的分子结构。基于目前的结果，讨论了一种可能的机制，这种机制似乎是这类缺失所特有的。
