含有大肠杆菌噬菌体λ粘性末端的质粒DNA的包装
Packaging of plasmid DNA containing the cohesive ends of coliphage lambda.
作者信息
Vollenweider H J, Fiandt M, Rosenvold E C, Szybalski W
出版信息
Gene. 1980 Apr;9(1-2):171-4. doi: 10.1016/0378-1119(80)90174-2.
PMID:6247245
Abstract
High yields of ColE1::Tn3-cos lambda plasmid genomes packaged in phage lambda virions (2.10(9) per ml) are produced by thermal induction of E. coli W3350 (lambda cI1857S7) lysogens carrying the plasmid DNA. The plasmid DNA is packaged in the linear form, with the right m' terminus of lambda being associated with the lambda tail.
摘要
通过热诱导携带质粒DNA的大肠杆菌W3350（λcI1857S7）溶原菌，可产生高产量的包装在λ噬菌体病毒粒子中的ColE1::Tn3-黏粒λ质粒基因组（每毫升2.1×10⁹个）。质粒DNA以线性形式包装，λ的右m'末端与λ尾巴相连。
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