Atkinson M M, Menko A S, Johnson R G, Sheppard J R, Sheridan J D
J Cell Biol. 1981 Nov;91(2 Pt 1):573-8. doi: 10.1083/jcb.91.2.573.
The transformed or normal phenotype of cultured normal rat kidney cells infected with a temperature-sensitive mutant of avian sarcoma virus is conditional on the temperature at which the cells are grown. Using dye injection techniques, we show that junction-mediated dye transfer is also temperature-sensitive. The extent and rate of transfer between infected cells grown at the transformation-permissive temperature (35 degrees C) is significantly reduced when compared to infected cells grown at the nonpermissive temperature (40.5 degrees C) or uninfected cells grown at either temperature. Infected cells subjected to reciprocal temperature shifts express rapid and reversible alterations of dye transfer capacities, with responses evident by 15 min and completed by 60 min for temperature shifts in either direction. These results suggest that altered junctional capacities may be fundamental to the expression of the ASV-induced, transformed phenotype.
感染了禽肉瘤病毒温度敏感突变体的培养正常大鼠肾细胞的转化或正常表型取决于细胞生长的温度。使用染料注射技术，我们发现连接介导的染料转移也是温度敏感的。与在非允许温度（40.5摄氏度）下生长的感染细胞或在任一温度下生长的未感染细胞相比，在转化允许温度（35摄氏度）下生长的感染细胞之间的转移程度和速率显著降低。经历双向温度变化的感染细胞表现出染料转移能力的快速且可逆的改变，对于任一方向的温度变化，在15分钟时反应明显，60分钟时完成。这些结果表明，连接能力的改变可能是ASV诱导的转化表型表达的基础。
