来自阴沟肠杆菌的限制性内切酶EcaI。

Hobom G, Schwarz E, Melzer M, Mayer H

Nucleic Acids Res. 1981 Oct 10;9(19):4823-32. doi: 10.1093/nar/9.19.4823.

Restriction endonuclease EcaI obtained from Enterobacter cloacae DSM30056 recognizes the group of heptanucleotide palindromes 5'-G[unk]G-T-N-A-C-C-3', and on cleavage (arrow) produces fragments with 5'-terminal pentanucleotide extensions. It is identical in specificity with restriction endonuclease BstEII from Bacillus stearothermophilus ET.

从阴沟肠杆菌DSM30056中获得的限制性内切酶EcaI识别七核苷酸回文序列5'-G[unk]G-T-N-A-C-C-3'，切割后（箭头所示）产生具有5'-末端五核苷酸延伸的片段。它与嗜热脂肪芽孢杆菌ET的限制性内切酶BstEII特异性相同。