编码Ecal DNA甲基转移酶的基因的克隆与核苷酸序列
Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase.
作者信息
Brenner V, Venetianer P, Kiss A
机构信息
Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged.
出版信息
Nucleic Acids Res. 1990 Jan 25;18(2):355-9. doi: 10.1093/nar/18.2.355.
The gene coding for the GGTNACC specific Ecal DNA methyltransferase (M.Ecal) has been cloned in E. coli from Enterobacter cloacae and its nucleotide sequence has been determined. The ecalM gene codes for a protein of 452 amino acids (Mr: 51,111). It was determined that M.Ecal is an adenine methyltransferase. M.Ecal shows limited amino acid sequence similarity to other adenine methyltransferases. A clone that expresses Ecal methyltransferase at high level was constructed.
编码针对GGTNACC特异性的埃卡尔肠杆菌DNA甲基转移酶（M.Ecal）的基因已从阴沟肠杆菌中克隆到大肠杆菌中，并测定了其核苷酸序列。ecalM基因编码一个由452个氨基酸组成的蛋白质（分子量：51,111）。已确定M.Ecal是一种腺嘌呤甲基转移酶。M.Ecal与其他腺嘌呤甲基转移酶的氨基酸序列相似性有限。构建了一个高水平表达埃卡尔甲基转移酶的克隆。
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