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编码Ecal DNA甲基转移酶的基因的克隆与核苷酸序列

Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase.

作者信息

Brenner V, Venetianer P, Kiss A

机构信息

Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged.

出版信息

Nucleic Acids Res. 1990 Jan 25;18(2):355-9. doi: 10.1093/nar/18.2.355.

DOI:10.1093/nar/18.2.355
PMID:2183182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330275/
Abstract

The gene coding for the GGTNACC specific Ecal DNA methyltransferase (M.Ecal) has been cloned in E. coli from Enterobacter cloacae and its nucleotide sequence has been determined. The ecalM gene codes for a protein of 452 amino acids (Mr: 51,111). It was determined that M.Ecal is an adenine methyltransferase. M.Ecal shows limited amino acid sequence similarity to other adenine methyltransferases. A clone that expresses Ecal methyltransferase at high level was constructed.

摘要

编码针对GGTNACC特异性的埃卡尔肠杆菌DNA甲基转移酶（M.Ecal）的基因已从阴沟肠杆菌中克隆到大肠杆菌中，并测定了其核苷酸序列。ecalM基因编码一个由452个氨基酸组成的蛋白质（分子量：51,111）。已确定M.Ecal是一种腺嘌呤甲基转移酶。M.Ecal与其他腺嘌呤甲基转移酶的氨基酸序列相似性有限。构建了一个高水平表达埃卡尔甲基转移酶的克隆。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3020/330275/3a1c2a6e3c94/nar00186-0134-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3020/330275/5e684e281cc0/nar00186-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3020/330275/cb3ee3012aaf/nar00186-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3020/330275/3a1c2a6e3c94/nar00186-0134-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3020/330275/5e684e281cc0/nar00186-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3020/330275/cb3ee3012aaf/nar00186-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3020/330275/3a1c2a6e3c94/nar00186-0134-b.jpg

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本文引用的文献

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Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
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Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.克隆的BamHI限制修饰系统的特性：其核苷酸序列、甲基化酶的性质以及在异源宿主中的表达。
Nucleic Acids Res. 1991 Feb 25;19(4):841-50. doi: 10.1093/nar/19.4.841.
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Genetic organization of the KpnI restriction--modification system.KpnI 限制 - 修饰系统的基因组织
Nucleic Acids Res. 1991 Dec 11;19(23):6505-9. doi: 10.1093/nar/19.23.6505.
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Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化：对DNA修饰甲基转移酶和限制性内切酶的影响。
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Cloning and expression of the HpaI restriction-modification genes.HpaI 限制-修饰基因的克隆与表达
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New M13 vectors for cloning.用于克隆的新型M13载体。
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The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.大肠杆菌DNA腺嘌呤甲基化酶（dam）基因的分离与鉴定。
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