Söchtig E, Trost T
Pharmacology. 1981;23(2):82-90. doi: 10.1159/000137532.
Eritadenine was investigated for its effects on adenylate cyclase activity of rat fat cell plasma membranes on cyclic AMP accumulation and lipolysis in isolated rat fat cells. In rat fat cell plasma membranes, a 50% inhibition of noradrenaline (100 mumol/l) stimulated adenylate cyclase was obtained at 11.6 mumol/l eritadenine or 9.0 mumol/l adenosine. NaF (3 mmol/l) stimulated adenylate cyclase was inhibited at concentrations of eritadenine (IC25 5.8 mumol/l) lower than those of adenosine (IC25 51.9 mumol/l). Eritadenine ethyl ester (100 mumol/l) was almost ineffective on adenylate cyclase. The inhibitory effect of eritadenine was resistant to adenosine deaminase. Isolated rat fat cells eritadenine (100 mumol/l) was completely ineffective to block noradrenaline-stimulated cyclic AMP accumulation or lipolysis stimulated by theophylline or adenosine deaminase. It is suggested that eritadenine is an effector of the adenosine P site of fat cell plasma membranes.
研究了香菇嘌呤对大鼠脂肪细胞质膜腺苷酸环化酶活性、对分离的大鼠脂肪细胞中环磷酸腺苷（cAMP）积累及脂肪分解的影响。在大鼠脂肪细胞质膜中，11.6 μmol/L香菇嘌呤或9.0 μmol/L腺苷可使去甲肾上腺素（100 μmol/L）刺激的腺苷酸环化酶活性受到50%的抑制。NaF（3 mmol/L）刺激的腺苷酸环化酶在香菇嘌呤浓度（IC25为5.8 μmol/L）低于腺苷（IC25为51.9 μmol/L）时受到抑制。香菇嘌呤乙酯（100 μmol/L）对腺苷酸环化酶几乎无作用。香菇嘌呤的抑制作用对腺苷脱氨酶有抗性。分离的大鼠脂肪细胞中，香菇嘌呤（100 μmol/L）完全无法阻断去甲肾上腺素刺激的cAMP积累或茶碱或腺苷脱氨酶刺激的脂肪分解。提示香菇嘌呤是脂肪细胞质膜腺苷P位点的效应物。
