Oehler J R, Lindsay L R, Nunn M E, Holden H T, Herberman R B
Int J Cancer. 1978 Feb 15;21(2):210-20. doi: 10.1002/ijc.2910210213.
Natural cell-mediated cytotoxicity in rats as well as in mice has been shown to vary consistently with age, with peak levels detectable at 5-10 weeks. The levels of cell-mediated cytotoxicity against tumor cells could be augmented in strains of inbred rats with either high or low levels of natural reactivity, by IP injection of a variety of agents, including C. parvum, LCMV, KRV, and poly I:C. The specificity of the augmented cytotoxicity appeared to be the same as the specificity of natural killer cells which are found in normal rat spleen cells. Similarly, the cells mediating the augmented cellular cytotoxicity were small, non-adherent, esterase-negative lymphocytes with Fc receptors, as are rat NK cells. The kinetics and organ distribution of the augmentation of NK activity by poly I:C and C. parvum were compared and the kinetics were found to differ, with a shorter time course of augmented activity seen after inoculation with poly I:C. These data indicate that interferon may play a central role in the augmentation of NK activity in vivo.
已证明，大鼠和小鼠体内的自然细胞介导的细胞毒性会随年龄持续变化，在5至10周时可检测到峰值水平。通过腹腔注射多种试剂，包括微小隐孢子虫、淋巴细胞脉络丛脑膜炎病毒、KRV和聚肌胞苷酸，在自然反应性水平高或低的近交系大鼠品系中，针对肿瘤细胞的细胞介导的细胞毒性水平均可提高。增强的细胞毒性的特异性似乎与正常大鼠脾细胞中发现的自然杀伤细胞的特异性相同。同样，介导增强的细胞毒性的细胞是具有Fc受体的小的、非黏附性、酯酶阴性淋巴细胞，大鼠自然杀伤细胞也是如此。比较了聚肌胞苷酸和微小隐孢子虫增强自然杀伤细胞活性的动力学和器官分布，发现动力学有所不同，接种聚肌胞苷酸后增强活性的时间进程较短。这些数据表明，干扰素可能在体内自然杀伤细胞活性增强中起核心作用。
