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[光诱导纤细裸藻细胞原生质膜电位的超极化]

[Light-induced hyperpolarization of membrane potential in Nitella flexilis call plasmalemma].

作者信息

Volkov G A

出版信息

Biofizika. 1981 Nov-Dec;26(6):1021-6.

PMID:6274428
Abstract

Light-induced hyperpolarization of the membrane potential in Nitella flexilis cell plasmalemma was investigated by the clamping method. It is shown that this response is of an excitation character. The equivalent electric diagram used in the work and the electromotive force included on its basis allowed consideration of two possible (from the common point of view) mechanisms of the response: the action of the electrogenic ion pump and membrane specific permeability to a definite ion. The membrane being in the hyperpolarization state is not capable of acting as a bicarbonate electrode. It is suggested that the response under study is defined by the action of the electrogenic pump, the role of which is performed by HCO2(3)-dependent H+-ATPase with the ion channel as an exciting one.

摘要

采用钳制方法研究了纤细裸藻细胞质膜中光诱导的膜电位超极化。结果表明,这种反应具有兴奋特性。工作中使用的等效电图及其基于此得出的电动势,使得可以从(一般观点)考虑两种可能的反应机制:生电离子泵的作用以及膜对特定离子的特异性通透性。处于超极化状态的膜不能作为碳酸氢盐电极。研究表明,所研究的反应是由生电泵的作用所定义的,其作用由依赖HCO₂(₃)⁻的H⁺ - ATP酶执行,离子通道作为激发通道。

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