Vuletić M, Vucinić Z, Radenović C
Gen Physiol Biophys. 1985 Apr;4(2):195-200.
The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An increase in the calcium concentration resulted in depolarisation, its magnitude increasing with lower proton concentrations. Calcium-induced membrane potential changes, tested in the concentration range of 0.25 mmol/l to 25 mmol/l, were greatest at 0.25 mmol/l CaCl2 and decreased with the increasing calcium concentration. Light-induced initial changes in the membrane potential also showed a dependence on the presence of calcium in the external medium. We conclude that calcium has a role in the regulation of the proton pump in Nitella.
轮藻细胞膜电位超极化超过扩散电位是由生电质子泵的作用所解释的。我们研究了钙与该泵功能的相互作用。使用标准微电极技术测量膜电位。钙浓度的增加导致去极化，其幅度随着质子浓度降低而增大。在0.25 mmol/l至25 mmol/l的浓度范围内测试的钙诱导的膜电位变化，在0.25 mmol/l CaCl2时最大，并随着钙浓度的增加而降低。光诱导的膜电位初始变化也显示出依赖于外部介质中钙的存在。我们得出结论，钙在丽藻属植物的质子泵调节中起作用。
