Zenou S M, Raichvarg D, Agneray J
Biomedicine. 1981 Oct;35(5):166-9.
A two-step method separating granulocytes and mononuclear cells from a single sample of 10 ml human blood is described. The technique combines a Ficoll-Hypaque treatment and a sedimentation in a Polyvinylic alcohol medium. This method (FHP) is then compared to the Ficoll-Hypaque-Dextran procedure. The FHP method requires approximately 2 h to be carried out and results in the preparation of granulocytes (greater than 99% purity) with high yield (40.7 % +/- 9.1). The cell viability exceeds 95%. Moreover the enzymic and phagocytic properties of polymorphonuclear cells are preserved after isolation. This method is then applied to study the influence of periodate treatment on nonspecific phagocytosis. In contrast with monocytes, we observed no decrease of phagocytic process.
本文描述了一种从10毫升人体血液的单个样本中分离粒细胞和单核细胞的两步法。该技术结合了Ficoll-Hypaque处理和在聚乙烯醇介质中的沉降。然后将此方法（FHP）与Ficoll-Hypaque-葡聚糖方法进行比较。FHP方法大约需要2小时完成，可高产量（40.7%±9.1）制备纯度大于99%的粒细胞。细胞活力超过95%。此外，多形核细胞的酶活性和吞噬特性在分离后得以保留。然后将该方法应用于研究高碘酸盐处理对非特异性吞噬作用的影响。与单核细胞不同，我们观察到吞噬过程没有下降。
