一种从全血中分离人粒细胞的快速一步法。
A rapid one-step method for the isolation of human granulocytes from whole blood.
作者信息
Jepsen L V, Skottun T
出版信息
Scand J Clin Lab Invest. 1982 May;42(3):235-8.
PMID:7134807
Abstract
A rapid one-step method for the separation of granulocytes, mononuclear cells and erythrocytes from peripheral blood using two different gradients of Percoll (density solution I: 1.075 kg/l; solution II: 1.096-1.098 kg/l) is described. The influence of temperature on the density of solution II was tested and the variations were found to be of some importance. The average yield of granulocytes from 14 donors was 87.6 +/- 9.6%, and the purity of the granulocytes was 99.66 +/- 0.33%.
摘要
描述了一种使用两种不同梯度的Percoll（密度溶液I：1.075 kg/l；溶液II：1.096 - 1.098 kg/l）从外周血中快速一步分离粒细胞、单核细胞和红细胞的方法。测试了温度对溶液II密度的影响，发现这些变化具有一定重要性。14名供体的粒细胞平均产量为87.6 +/- 9.6%，粒细胞纯度为99.66 +/- 0.33%。
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