Hara A, Fukuyama K, Epstein W L
Clin Chim Acta. 1981 Dec 24;117(3):269-77. doi: 10.1016/0009-8981(81)90114-5.
Activities of angiotensin-converting enzyme and acid phosphatase were markedly elevated in the lesions of metal-induced hypersensitivity and foreign body types of granulomas. We biochemically studied these enzymes by means of gel filtration and electrophoresis and compared them with the same enzymes of normal dermis. Two forms of angiotensin-converting enzyme found in normal dermis were increased proportionally. Catalytic properties and heat stability of the enzyme in the lesions and normal dermis were the same. On the other hand, acid phosphatase in the lesions showed a different isozyme pattern from that seen in normal dermis. The enzyme from normal dermis had apparent molecular weight of 150000 and 90000, whereas an additional enzyme with a molecular weight of 230000 was detected in granulomatous lesions. Three isozymes were demonstrable in normal dermis but two additional isoenzymes appeared in granulomatous lesions: the isozyme patterns between the foreign body and hypersensitivity granulomas are different.
在金属诱导的超敏反应性病变和异物型肉芽肿中，血管紧张素转换酶和酸性磷酸酶的活性显著升高。我们通过凝胶过滤和电泳对这些酶进行了生化研究，并将它们与正常真皮中的相同酶进行了比较。正常真皮中发现的两种血管紧张素转换酶形式成比例增加。病变组织和正常真皮中该酶的催化特性和热稳定性相同。另一方面，病变组织中的酸性磷酸酶显示出与正常真皮不同的同工酶模式。正常真皮中的酶表观分子量为150000和90000，而在肉芽肿性病变中检测到一种分子量为230000的额外酶。正常真皮中可显示三种同工酶，但在肉芽肿性病变中出现另外两种同工酶：异物肉芽肿和超敏反应性肉芽肿之间的同工酶模式不同。
