Tn3转座酶蛋白的氨基末端序列。
Amino-terminal sequence of the Tn3 transposase protein.
作者信息
Ditto M D, Chou J, Hunkapiller M W, Fennewald M A, Gerrard S P, Hood L E, Cohen S N, Casadaban M J
出版信息
J Bacteriol. 1982 Jan;149(1):407-10. doi: 10.1128/jb.149.1.407-410.1982.
Abstract
The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.
摘要
Tn3转座酶蛋白的氨基末端序列被确定为脯氨酸-缬氨酸-天冬氨酸-苯丙氨酸-亮氨酸-苏氨酸-苏氨酸-谷氨酸-谷氨酰胺-缬氨酸-谷氨酸-丝氨酸……这是通过从转座酶高产突变菌株中纯化的活性转座酶蛋白以及通过杂合转座酶-β-半乳糖苷酶融合蛋白确定的。该氨基酸序列与转座酶基因从ATG起始密码子开始的DNA序列相对应，正如先前通过转座酶-β-半乳糖苷酶基因融合分析所预测的那样。
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