Nishiya I, Nunokawa S, Saitoh S, Ishizaki Y, Yamashita K
Nihon Sanka Fujinka Gakkai Zasshi. 1981 Nov;33(11):1910-6.
Treatment of choriocarcinoma should be eradicated of trophoblastic cells which undergo destruction in both of primary and metastatic sites and Methotrexate (MTX) has a most effective value. While MTX is being administration to the patients, there is no determination of clear relationship between proliferation of trophoblastic cells and production of human chorionic gonadotropin (hCG). In this report, we attempted to clarify the changes of the dividing compartments in cell cycle of human choriocarcinoma in vitro in the administration of MTX (concentration in 5 x 10(-6) M and 5 x 10(-7) M) to the experimental cells. Cell samples were stained by propidium iodide (PI) and fluorescein isothiocyanate (FITC), then were measured nucleic acid and protein content by means of flow microfluorometry (FMF). MTX was remarkably increased the intensity of FITC associated with protein production, in dose that is not only killing effect to the cells but inhibition of DNA synthesis. Moreover, the most difference in both subjects were as follows; Ever when MTX in low concentration was removed after 48 hours, 50% of experimental cells recovered to the level of controls in contrast to the persistence of remarkable inhibition of DNA synthesis in high concentration.
绒毛膜癌的治疗应根除滋养层细胞，这些细胞在原发部位和转移部位均会遭到破坏，而甲氨蝶呤（MTX）具有最有效的治疗价值。在给患者使用MTX时，滋养层细胞增殖与人类绒毛膜促性腺激素（hCG）产生之间的明确关系尚未确定。在本报告中，我们试图阐明在向实验细胞施用MTX（浓度为5×10⁻⁶ M和5×10⁻⁷ M）时，人绒毛膜癌体外细胞周期中分裂区室的变化。细胞样本用碘化丙啶（PI）和异硫氰酸荧光素（FITC）染色，然后通过流式细胞荧光术（FMF）测量核酸和蛋白质含量。MTX显著增加了与蛋白质产生相关的FITC强度，该剂量不仅对细胞有杀伤作用，还抑制DNA合成。此外，两个实验组的最大差异如下：即使在48小时后去除低浓度的MTX，50%的实验细胞恢复到对照水平，而高浓度的MTX则持续显著抑制DNA合成。
