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甲氨蝶呤对培养的绒毛膜癌细胞中人绒毛膜促性腺激素和胎盘碱性磷酸酶的刺激作用。

The stimulation by methotrexate of human chorionic gonadotropin and placental alkaline phosphatase in cultured choriocarcinoma cells.

作者信息

Speeg K V, Azizkhan J C, Stromberg K

出版信息

Cancer Res. 1976 Dec;36(12):4570-6.

PMID:1087181
Abstract

Treatment of the BeWo line of choriocarcinoma cells with methotrexate in doses that inhibit DNA synthesis causes a tenfold increase in synthesis of human chorionic gonadotropin and a threefold increase in activity of placental alkaline phosphatase. No concomitant increase in lactic dehydrogenase activity occurs under these conditions. This effect of methotrexate can be blocked by simultaneous addition of thymidine or folinic acid, neither of which alone increases human chorionic gonadotropin synthesis or placental alkaline phosphatase activity in BeWo cells.

摘要

用抑制DNA合成剂量的甲氨蝶呤处理绒癌BeWo细胞系，会导致人绒毛膜促性腺激素的合成增加10倍，胎盘碱性磷酸酶的活性增加3倍。在这些条件下，乳酸脱氢酶活性没有随之增加。甲氨蝶呤的这种作用可通过同时添加胸苷或亚叶酸来阻断，单独使用这两种物质均不会增加BeWo细胞中人绒毛膜促性腺激素的合成或胎盘碱性磷酸酶的活性。

相似文献

1
The stimulation by methotrexate of human chorionic gonadotropin and placental alkaline phosphatase in cultured choriocarcinoma cells.甲氨蝶呤对培养的绒毛膜癌细胞中人绒毛膜促性腺激素和胎盘碱性磷酸酶的刺激作用。
Cancer Res. 1976 Dec;36(12):4570-6.
2
Stimulation of human chorionic gonadotropin by JAr line choriocarcinoma after inhibition of DNA synthesis.DNA合成抑制后JAr系绒毛膜癌对人绒毛膜促性腺激素的刺激作用
Cancer Res. 1979 Jun;39(6 Pt 1):1952-9.
3
Inhibition of methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells by thymidine.
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A human gastric choriocarcinoma cell line with human chorionic gonadotropin and placental alkaline phosphatase production.一种能产生人绒毛膜促性腺激素和胎盘碱性磷酸酶的人胃绒毛膜癌细胞系。
Cancer Res. 1975 Aug;35(8):2025-32.
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Density-dependent inhibition of expression of syncytiotrophoblastic markers by cultured human choriocarcinoma (BeWo) cells.
J Cell Physiol. 1986 Sep;128(3):375-82. doi: 10.1002/jcp.1041280305.
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Effects of adenosine cyclic nucleotides on the synthesis of human chorionic gonadotropin in transformed human placental cells.腺苷环化核苷酸对转化的人胎盘细胞中人绒毛膜促性腺激素合成的影响。
Cancer Res. 1980 Nov;40(11):4025-30.
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Differential regulation of choriocarcinoma gene expression by DNA synthesis inhibitors.
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8
Differential modulation of human chorionic gonadotropin production by methotrexate in normal and malignant placental cultures and its increase by dibutyryl cyclic adenosine monophosphate and/or actinomycin D in normal cultures.甲氨蝶呤对正常及恶性胎盘培养物中人绒毛膜促性腺激素产生的差异调节及其在正常培养物中被二丁酰环磷腺苷和/或放线菌素D增强。
Cancer Res. 1987 Jan 15;47(2):383-7.
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[Specific effects of methotrexate bound to the antibodies against hCG and placental alkaline phosphatase to the cultured human choriocarcinoma cells].[甲氨蝶呤与抗人绒毛膜促性腺激素及胎盘碱性磷酸酶抗体结合对培养的人绒毛膜癌细胞的特异性作用]
Nihon Sanka Fujinka Gakkai Zasshi. 1983 Jan;35(1):33-40.
10
[Effect of methotrexate on cell growth and the production of hCG, beta-hCG and SP-1 in cultured choriocarcinoma cell lines].[甲氨蝶呤对培养的绒毛膜癌细胞系中细胞生长及人绒毛膜促性腺激素、β-人绒毛膜促性腺激素和妊娠相关血浆蛋白-A产生的影响]
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Jan;36(1):13-20.

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Biochem J. 1993 Jan 1;289 ( Pt 1)(Pt 1):101-7. doi: 10.1042/bj2890101.
3
Effects of hydroxyurea and cyclic adenosine monophosphate/protein kinase A inhibitors on the expression of the human chorionic gonadotropin alpha subunit and c-myc genes in choriocarcinoma.
羟基脲和环磷酸腺苷/蛋白激酶A抑制剂对绒毛膜癌中人绒毛膜促性腺激素α亚基和c-myc基因表达的影响
J Endocrinol Invest. 1993 Dec;16(11):849-56. doi: 10.1007/BF03348942.
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Alkaline phosphatase protein increases in response to prednisolone in HeLa cells.在HeLa细胞中，碱性磷酸酶蛋白会因泼尼松龙而增加。
Biochem J. 1981 Nov 15;200(2):461-4. doi: 10.1042/bj2000461.
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Enhancement of human chorionic gonadotrophin production by antimetabolites.抗代谢物对人绒毛膜促性腺激素产生的增强作用。
Br J Cancer. 1982 Jul;46(1):22-9. doi: 10.1038/bjc.1982.160.
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Sodium butyrate stimulates hCG and hCG-alpha production in a human choriocarcinoma cell line (BeWo).丁酸钠可刺激人绒毛膜癌细胞系（BeWo）中hCG和hCG-α的产生。
J Cancer Res Clin Oncol. 1981;100(3):321-4. doi: 10.1007/BF00410692.
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