[3H]地西泮在人脑脊液中的置换活性。

[3H]Diazepam displacing activity in human cerebrospinal fluid.

作者信息

Kuhn W, Neuser D, Przuntek H

出版信息

J Neurochem. 1981 Oct;37(4):1045-7. doi: 10.1111/j.1471-4159.1981.tb04494.x.

DOI:10.1111/j.1471-4159.1981.tb04494.x

Abstract

Pooled human cerebrospinal fluid was separated by Sephadex G-50 chromatography. The presence of three peaks, A, B and C, was demonstrated by monitoring absorbance at 254 and 280 nm. All peaks showed [3H]diazepam displacing activity in the membrane receptor test. Peak B was further separated on Bio-Gel P-4. At least two major fractions free of salt and GABA in the molecular weight range of approximately 700--3600 were shown to displace [3H]diazepam in the receptor test. This activity was enhanced by a factor of 3 in the presence of 10 microM-GABA.

摘要

收集的人脑脊液通过Sephadex G - 50柱色谱法进行分离。通过监测254和280nm处的吸光度，证实存在三个峰，即A、B和C峰。在膜受体试验中，所有峰均显示出[³H]地西泮置换活性。峰B在Bio - Gel P - 4上进一步分离。在受体试验中，显示出至少两个主要的无盐且分子量范围约为700 - 3600的不含γ-氨基丁酸（GABA）的级分能够置换[³H]地西泮。在10μM - GABA存在下，这种活性增强了3倍。