Franco R, Canela E I, Bozal J
Rev Esp Fisiol. 1981 Sep;37(3):255-62.
In soluble rat brain fraction, the specific activities of purine nucleoside phosphorylase, guanine deaminase, 5'Nucleotidase and adenosine deaminase, decrease in their mentioned order. A kinetic parameter comparison between these enzymes shows that 5'Nucleotidase with AMP has the lowest KM and the greatest Vmax values, while purine nucleoside phosphorylase has its lowest KM and its greatest Vmax values with guanosine and with inosine, respectively. The enzymes activity is not modified by the metabolic intermediates differently from their own reaction products which behave as competitive inhibitors.
在可溶性大鼠脑匀浆中，嘌呤核苷磷酸化酶、鸟嘌呤脱氨酶、5'-核苷酸酶和腺苷脱氨酶的比活性按上述顺序降低。这些酶之间的动力学参数比较表明，5'-核苷酸酶与AMP反应时具有最低的KM值和最大的Vmax值，而嘌呤核苷磷酸化酶分别与鸟苷和肌苷反应时具有最低的KM值和最大的Vmax值。与作为竞争性抑制剂的自身反应产物不同，代谢中间产物不会改变这些酶的活性。
