Poplack D G, Blatt J, Reaman G
Cancer Res. 1981 Nov;41(11 Pt 2):4821-23.
The status of three purine pathway enzymes, adenosine deaminase, 5'-nucleotidase, and purine nucleoside phosphorylase, was evaluated in the leukemic cells of patients with acute lymphoblastic leukemia and correlated with routine immunological cell surface markers. A distinct pattern of enzyme activity was noted in T-lymphoblasts which have significantly higher adenosine deaminase activity (p less than 0.02) and lower 5'-nucleotidase (p less than 0.001) and purine nucleoside phosphorylase (p less than 0.01) activities than do non-T, non-B lymphoblasts. This enzyme pattern is similar to that observed in normal human thymocytes but is not shared by the mature, normal T-lymphocytes of peripheral blood, suggesting that it may reflect the differentiation status of malignant T-lymphoblasts. These findings, which confirm the biochemical heterogeneity of acute lymphoblastic leukemia, may provide an avenue for selective chemotherapy of this disease.
对急性淋巴细胞白血病患者白血病细胞中的三种嘌呤代谢途径酶，即腺苷脱氨酶、5'-核苷酸酶和嘌呤核苷磷酸化酶的状态进行了评估，并与常规免疫细胞表面标志物进行了相关性分析。在T淋巴细胞母细胞中观察到一种独特的酶活性模式，其腺苷脱氨酶活性显著高于非T、非B淋巴细胞母细胞（p<0.02），而5'-核苷酸酶（p<0.001）和嘌呤核苷磷酸化酶（p<0.01）活性则低于非T、非B淋巴细胞母细胞。这种酶模式与正常人胸腺细胞中观察到的相似，但外周血成熟正常T淋巴细胞不具有，这表明它可能反映了恶性T淋巴细胞母细胞的分化状态。这些发现证实了急性淋巴细胞白血病的生化异质性，可能为该疾病的选择性化疗提供途径。
